Abstract:
Somatic erabryogenesis is an efficient regeneration system with high genetic integrity ayich useful in tea crop improvement. The prosent study was conducted with the objective of developing a viable somatic embryogenic protocol from leaf calli of tea. Leaf segments of z ^ (el) leaves of FRI 2043 and 2024 cultivars were used to indsce calli and for induction of somatic embryos 2nd and 3 ^ prime prime sub cultures were selected. Calli were inoculated in MS media with (1) 2mg * l ^ - 1 benzyl amino purine + 3 mgt naphthol acetic acid; and (ii) 2mg * l ^ - 1 benzyl amino purine - 3.5me * l ^ - 1 naphthol acetic acid growth regulator combinations. The greatest amount of embryogenic callus proliferation in both cultivars was achieved from 3 ^ prime prime subcultures using 2mgl - 1 benzyl amino oumise+ 3.5me * l ^ - 2 naphthol acetic acid medium. Compact and friable callus was observed in all culture bottles 3 weeks after culturing and friable calli was reported as the best for somatic embryo induction. Somatic embryoids were observed in 2mg * l ^ - 1 benzyl amino rho urine+ 3mz * l ^ - 1 naphthol acetic acid of TRI 2043. Significantly highost relative growth rate (92.21 %) was observed from leaf callus in 2mo * t ^ 4 benzyl amino perine+ 3.5 3.5mg * l ^ - 1 naphthol acetic acid of TRI 2024 when, using 2 subculture (Mean value 83.89) second subculture of leaf callus in MS medium with 2mx * l ^ 4 benzyl amino purine 3 mathfrak mgt ^ (- t) naphthol acetic acid is better to indice somatic embryos
